N-Alkylpyridinium sulfonates as versatile scout compounds for multi-mode highly targeted metabolomics: Application to Gammarus fossarum.
Although targeted metabolomics using multiple reaction monitoring (MRM) offers high sensitivity, it struggles with multiplexing capacity and retention time drifts. Scout-triggered MRM (stMRM) overcomes these limitations by using reference molecules to dynamically frame the acquisition window. However, establishing comp...