Rapid and Femtogram-level Sensitive Detection of Blastocystis spp. Using Colorimetric and Fluorometric LAMP Assays: Analytical and Clinical Validation.
BACKGROUND/AIM Blastocystis spp. is a genetically diverse protozoan parasite, often underdiagnosed due to low sensitivity and technical issues of current diagnosis techniques, wet mount microscopy and PCR. To bridge this gap, we developed loop-mediated isothermal amplification (LAMP) platforms, namely colorimetric (cLA...