A. Lewis

Benchmarking cell segmentation methods for cardiac spatial transcriptomics using membrane staining as ground truth

Imaging-based spatial transcriptomics can resolve gene expression at subcellular level in intact tissue, but its value in human myocardium depends on accurate cell segmentation. Adult cardiomyocytes are large (100–150 µm), elongated, and often multinucleated; they bear no resemblance to the small round cells that...