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Jul 2026

A systematic evaluation of the sources of non-uniform coverage in RNA-Seq and limitations of current practices.

It is demonstrated that no single factor can explain the non-uniformity in coverage across transcripts in RNA-Seq and that a connection of coverage with PCR that depends more on molecular abundance than PCR cycle number is demonstrated.

Thomas G. Brooks, N. Lahens, Antonijo Mrcela et al. · 0 citations

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