Rapid detection of Streptococcus agalactiae using an RPA-MbCas12a CRISPR assay targeting novel sodA and groL markers
Streptococcus agalactiae (Group B Streptococcus, GBS) is a major cause of neonatal and invasive infections, necessitating rapid and sensitive diagnostic approaches beyond conventional culture and PCR. Here, we developed an isothermal recombinase polymerase amplification (RPA)-MbCas12a CRISPR assay for fluorescence-base...