Akira K Nakamura

Cas3-mediated genome reduction: demonstration in Cupriavidus necator H16 improves growth on heterotrophic and autotrophic carbon sources

A Cascade–Cas3-enabled method called TRIM3 is reported that generates large deletions by targeting a randomly integrated transposon, enabling facile generation of a genome-reduced mutant library and represents a new avenue for large-scale genome modifications and the development of improved bioprocessing hosts.