An-Dong Li

A second-sphere variant of cytochrome P450 PbdA improves turnover and apparent coupling in veratrate oxidation

Efficient use of reducing equivalents is critical for productive turnover in cytochrome P450 redox enzymes, yet improving activity without compromising substrate affinity remains challenging. Here, we engineered the second-sphere region of PbdA, a cytochrome P450 from Rhodococcus jostii RHA1 that catalyzes veratrate ox...