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Sponsored Artificial Intelligence: A Modern Approach, Global Edition Artificial Intelligence: A Modern Approach, Global Edition

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Andonita Ajdari

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Open access Aug 2026

A synthetic biology approach to bacterial transcription initiation: RNA aptamer based in vitro transcription assay for rapidly testing bacterial RNA polymerases, promoters and inhibitors

We present a robust and versatile in vitro transcription (IVT) assay based on an optimized Broccoli RNA aptamer sequence. When paired with the fluorophore DFHBI-1T, this system enables real-time monitoring of multi-round transcription over several hours. To facilitate streamlined promoter analysis, we developed the pIV...

Tina Lanzmaier, Elena Reiterer, Melanie Merl et al. · 0 citations

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