Enhancing the activity of the bifunctional membrane Δ12-hydroxylase/desaturase CpFAH12: Key insights from redox partner interactions and electron transfer pathways.
Fatty acid desaturases introduce double bonds into fatty acyl chains to produce unsaturated fatty acids. Some membrane-bound desaturases also catalyze hydroxylation, yet the mechanistic link between desaturation and hydroxylation in bifunctional enzymes remains unresolved. Here, we combined molecular modelling and expe...