Mechanistic Insights into Poloxamer 188-Mediated Stabilization of AAV8 Capsids Using Coarse-Grained Molecular Dynamics.
PURPOSE Aggregation of adeno-associated virus (AAV) during manufacturing and storage remains a challenge in gene therapy formulation. This study evaluates how varying Poloxamer188 (P188) concentrations affect the structural stability and aggregation behavior of two full AAV8 capsids. METHODS Coarse-grained molecular...