Expression, Purification, and Structural Characterization of Membrane Proteins: A Case Study of the ABC Transporter MsbA.
This chapter outlines a detailed workflow for the expression, purification, and structural analysis of the ABC transporter MsbA from Pseudomonas aeruginosa (PaMsbA), which exemplifies how biochemical optimization, native mass spectrometry, and cryo-electron microscopy (cryo-EM) can be integrated to investigate membrane...