Open access
Aug 2026
LUCas: Light-Uncaged Cas13a using photocleavable interfering guide RNAs
Abstract CRISPR diagnostics enable sensitive detection of infectious diseases, with the RNA endonuclease Cas13a providing specific, amplification-free RNA detection through collateral trans-cleavage of fluorescent reporters. However, background cleavage from unbound enzyme, contaminating nucleases, and unsynchronized i...
Carlos F. Ng, Deepak Krishnamurthy, Andres Dextre et al.
· Nucleic Acids Research · 0 citations