Assessment of Unbound Rebastinib in Human Plasma and Serum Albumin by Equilibrium Dialysis Coupled with High-resolution Orbitrap Mass Spectrometry
Plasma protein binding (PPB) is a critical determinant of drug pharmacokinetics, as only the unbound fraction (fᵤ) of a drug is available for tissue distribution, target engagement, and clearance. However, the interaction of rebastinib with plasma proteins has not been fully elucidated to date. In this study, the free...