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B. Desguin

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Open access Aug 2026

Structural and functional analysis of LarC, a CTP-dependent cyclometallase required for nickel-pincer nucleotide cofactor biosynthesis

Biosynthesis of the nickel-pincer mononucleotide metallocofactor requires a CTP-dependent nickel insertion reaction catalyzed by LarC, whose mechanism of C-Ni bond formation is not fully understood. Here, we report the first cryo–electron microscopy structures of full-length LarC from Moorella thermoacetica with and wi...

Robert Wolfe, Aiko Turmo, Samantha Velasquez-Rivertte et al. · 0 citations
Review Open access Sep 2026

One cofactor, many enzymes: mechanistic and functional insights into nickel-pincer nucleotide-dependent biochemistry.

Nickel-pincer nucleotide (NPN) cofactors represent a groundbreaking class of organometallic cofactors in which a nickel ion is embedded within a tridentate pincer ligand derived from a modified nucleotide. Initially discovered in lactate racemase (LarA), NPN cofactors have since been identified in three distinct enzyme...

B. Desguin · 0 citations

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