2026
Gene Silencing by CRISPR Interference in Fusobacterium nucleatum.
Shiqi Xu, B. G. C., Kexin Tan et al.
· Methods in molecular biology · 0 citations
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This chapter provides a detailed, step-by-step protocol for implementing a conditional plasmid system that enables efficient, markerless gene deletion in FNA strains and provides a powerful and adaptable tool for advancing genetic studies in this genetically recalcitrant subspecies.