Author

B. Ingirbay

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Open access Aug 2026

Scalable Production of Transfection-Grade Plasmid DNA by Liquid–Liquid Extraction Without Chromatography: Application to CAR-T Vector Packaging

The acidic phenol extraction method is a robust, scalable, and cost-effective alternative to industry-standard methods, matching them in yield and purity while being readily scalable, making it a practical tool for both academic research and preparative laboratory production.

Gaziza Nigmatulla, Aitolkyn Kydyrbayeva, T. Kulatay et al. · Bioengineering · 0 citations

Open access Jul 2026

Suspension-Adapted HEK293FT Cells Enable High-Density Transfection for Efficient Lentiviral Vector Production in CAR-T Therapy

The platform based on suspension-adapted HEK293FT-DS cells enables high-density transfection and provides a cost-effective, scalable alternative to commercial LV production systems, particularly suited for academic CAR-T cell manufacturing.

A. Shevtsov, Aitolkyn Kydyrbayeva, Gaziza Nigmatulla et al. · Bioengineering · 0 citations