Methanol-Free Production of Recombinant AppA Phytase in Komagataella phaffii: Evaluation of Cultivation Conditions, Bioreactor Cultivation, and Biochemical Characterization
Phytases are widely used as feed additives because they improve phosphorus utilization from plant-derived feed ingredients and reduce phosphorus excretion into the environment. Most recombinant AppA phytases produced in Komagataella phaffii have been obtained using methanol-inducible expression systems, whereas informa...