Open access
Aug 2026
A FRET Ligation Assay using Fluorescent Proteins for Bacterial Sortase Enzymes
Bacterial sortases are widely used in sortase-mediated ligation (SML) experiments for various protein engineering applications. The power of these enzymes to bind and cleave a specific recognition motif, followed by ligation to another substrate using a ping-pong reaction mechanism has numerous applications in vaccine...
Adam Wachsman, Erich G. Walkenhauer, Kaia Stover et al.
· bioRxiv · 0 citations