The Alpha-CRISPR RNA detection system developed in this study integrates AlphaLISA technology with the CRISPR-Cas13a system, achieving significantly enhanced detection sensitivity, demonstrating substantial application potential in fundamental medical research and clinical diagnostics.
Yaofan Lu, Zhen-Ya Cheng, Zheng Wang et al.· ACS Sensors· 0 citations
This study identifies that upon the elevation of intracellular c-di-GMP levels induced by antibiotic treatmen, the universal stress protein UspG serves as a cryptic, direct c-di-GMP effector in extended-spectrum beta-lactamase (ESBL)-producing strain K. pneumoniae, and indicates that UspG is functionally conserved acro...
Xiao-Xiao Liu, Mingfang Wang, Yi-Kai Fu et al.· PLoS Pathogens· 0 citations
This programmable dual-aptamer amplified luminescent proximity homogeneous assay (AlphaLISA) provides a rapid, homogeneous, and highly adaptable strategy for tumor-derived sEVs analysis, offering promising potential for noninvasive cancer diagnostics.