Enzymatic Preparation and Regeneration of Protoplasts from the Red Microalga Porphyridium purpureum
Gene editing techniques are well-developed for model microalgae like Chlorella and Chlamydomonas. However, for the high-value species Porphyridium purpureum, its thick cell wall poses a significant barrier to genetic manipulation. Overcoming this challenge requires a robust method for preparing highly viable protoplast...