Author

Boonfei Tan

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Review Open access Sep 2026

Phylogenomic survey of GTDB r232 expands the taxonomic and ecological range of alkylsuccinate synthase and distinguishes divergent AssA-like paralogs

Alkylsuccinate synthase (AssA / MasD) catalyses fumarate-dependent anaerobic alkane activation. Characterised enzymes are confined to a few cultured sulfate reducers, nitrate-reducers and the archaeon Archaeoglobus fulgidus, and each acts over a narrow carbon-number range. Sequence similarity alone does not separate As...

Boonfei Tan, Christian Zafra, C. Ng · Scientific Reports · 0 citations

Open access Jan 2026

Comparative genomics of the Smithellaceae: reductive evolution and a mobile alkane-activation module in Smithella

Abstract Smithella are repeatedly implicated as primary alkane activators in methanogenic hydrocarbon-degrading communities, yet the propionate-oxidizing type species and many relatives cannot activate alkanes, and the genus’s diversity and functional differentiation remain poorly resolved. Here, we present genus-scale...

Boonfei Tan, Christian Zafra, C. Ng · ISME Communications · 0 citations

Open access Jul 2026

Comparative genomics of the Nap2-2B clade reveals substrate partitioning and niche diversification among uncultured hydrocarbon-degrading Desulfotomaculales.

Analysis of uncultured Nap2-2B bacteria genomes supports a syntrophic guild structured by substrate partitioning, putative cofactor complementation, and distinct electron-disposal strategies that may shape methanogenic hydrocarbon attenuation in anoxic tailings environments.

Boonfei Tan, Christian Zafra, C. Ng · Scientific Reports · 0 citations