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Author

Bridgie Cawthon

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Open access Oct 2026

Adaptive rewiring of NADPH electron flow reveals escape routes from redox-balance-based growth coupling in Escherichia coli.

Redox-balance-based growth coupling links oxidoreductase activity to cellular fitness and can enable high-throughput enzyme selection and strain evolution. However, because growth reflects the fitness of the whole cell, metabolic adaptation can circumvent the intended selective pressure. Here, we investigated the evolu...

Aidan E. Cowan, Mason Hillers, De-Tong Wu et al. · 0 citations
Open access Oct 2026

Replacing anaerobic fermentation with reverse β-oxidation enables growth-coupled evolution for enhanced fatty-alcohol production

Engineering robust fermentation hosts remains a barrier to scalable biomanufacturing. In this work we replace canonical mixed-acid fermentation in Escherichia coli with a redox‑balanced reverse β‑oxidation (rBOX) pathway that produces medium‑chain fatty alcohols and is bioenergetically equivalent to ethanol ferme...

Bridgie Cawthon, Aidan E. Cowan, Zen Yoshikawa et al. · 0 citations

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