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Author

Bruno Stegani

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Open access Oct 2026

Calcium-Driven Activation of Gelsolin: Mechanism and the Active-State Ensemble

Gelsolin is a master regulator of the actin cytoskeleton whose activity is controlled by large Ca2+-dependent conformational transitions of its 6 domains (G1-G6). Despite decades of biochemical and cellular studies, the structural basis of activation of the full-length protein remains poorly understood because active g...

Bruno Stegani, Loic Girois, Federico Ballabio et al. · 0 citations
Open access Sep 2026

Assessing multi-eGO Predictions of PDZ2–Peptide Binding across Mutations

The results indicate that the simplified energy landscape of multi-eGO limits the quantitative prediction of absolute kinetics while preserving thermodynamic information relevant to relative binding affinity, establishing multi-eGO as a computationally efficient approach for protein–peptide recognition and for predicti...

Camilla Ardizzone, Bruno Stegani, Fran Bačić Toplek et al. · 0 citations

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