A low-input, automated CUT&RUN workflow that incorporates standardized sample preparation to ensure reliable generation of data at the consortium scale is developed and validated and establishes a scalable, high-resolution platform for profiling chromatin landscapes from minimal cell inputs.
Aaron J. Alcala, M. Marunde, C. L. Windham et al.· Journal of Immunology· 0 citations
An integrated single-molecule view of regulatory remodeling may support identification of adaptive resistance mechanisms, compensatory regulatory programs, pharmacodynamic biomarkers, and candidate synthetic lethal interactions relevant to epigenetic drug development.
Keith E. Maier, James T. Anderson, Connor P. Frasier et al.· Clinical Cancer Research· 0 citations
A detailed CUT&RUN protocol from sample collection through data analysis, including best practices and defined controls to ensure specific, efficient, and robust target profiling is provided.
Tessa M. Firestone, Bryan J. Venters, Katherine Novitzky et al.· Methods in molecular biology· 0 citations
Fiber-seq simultaneously profiles chromatin accessibility, DNA methylation, protein footprints, and genetic variation on single molecules at near—base-pair resolution, revealing how genetic and epigenetic features interact to regulate gene expression and provides a powerful new framework for dissecting immune cell func...
Emily A. Madden, James T. Anderson, M. Cowles et al.· Journal of Immunology· 0 citations
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