Author

Bungo Akiyoshi

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Open access Sep 2026

Auxin-inducible degron-mediated protein depletion in Diplonema papillatum

Diplonemids are highly abundant unicellular flagellates that are evolutionarily divergent from traditional model eukaryotes. Diplonema papillatum is a model diplonemid for which several molecular tools have recently been established. However, no method for conditional protein depletion was available. Here, we have adap...

Gillian Clifford, Bungo Akiyoshi · bioRxiv · 0 citations

Open access Aug 2026

The centromere localization domain of kinetoplastid kinetochore protein KKT2 recognizes the free N-terminus of histone H3

Kinetochores are multiprotein complexes that drive chromosome segregation in eukaryotes. Kinetoplastids, a group of early-diverging eukaryotes that lack canonical kinetochore components, have a unique set of kinetochore proteins. How their kinetochores are assembled specifically at centromeres in the absence of a centr...

Aleksandra Ciszek, Patryk Ludzia, G. Marcianò et al. · bioRxiv · 0 citations