Single-protein diffusion in micrometer-size vesicles is governed by viscosity, surface charge, and membrane interactions
Giant unilamellar vesicles (GUVs) are a powerful model system for studying protein dynamics in cell-sized compartments with a precisely tunable physicochemical environment. Here, we apply Single-Molecule Displacement Mapping (SMdM) to quantify how luminal viscosity, ionic strength, membrane lipid composition, and prote...
Jelmer Coenradij, Dmitrii Linnik, Kristine Postmus et al.
· bioRxiv · 0 citations