Can Liu

LNA Blocker-Enhanced Multiplex Chip-Based Digital PCR for Ultrasensitive Quantification of Liver-Specific DNA Methylation in Plasma

Sensitive and selective quantification of rare methylated DNA in plasma remains a major analytical challenge. For methylation targets located in CpG-sparse regions, bisulfite conversion produces only subtle sequence differences between methylated and unmethylated alleles, thereby hindering selective amplification. He...