Establishment of a genome editing system and expression of heterologous proteins in the nonconventional yeast Pichia kudriavzevii
Impact statement Nonconventional yeast Pichia kudriavzevii possesses attractive phenotypic traits for industrial biomanufacturing; however, it remains genetically intractable compared with established yeast chassis. Here, we present an integrated synthetic biology framework that renders P. kudriavzevii a programmable p...