Cryptic Methylation Enables Amino-Maleimide Formation in Ligiamycin Biosynthesis
Maleimide is a rare but powerful pharmacophore in natural products, yet its biosynthetic logic remains poorly understood. Here, we elucidate the biosynthesis of ligiamycins, bacterial decalin–amino-maleimide metabolites from Streptomyces sp. NA07271. Genetic and biochemical studies revealed an unusual dispersed PKS–N...