Chengcheng Jiang

A product-determining structural loop in a novel PL40 ulvan lyase controls oligosaccharide profile.

It is shown that variations in the architecture of the substrate-binding cleft dictate whether these enzymes generate disaccharides or longer oligosaccharides as their dominant products, helping to understand how PL40 enzymes work and offering a starting point for designing ulvan lyases that make specific products.