Multiplex digital PCR for rapid quantitative detection and dynamic monitoring of respiratory pathogens in bronchoalveolar lavage fluid.
Lower respiratory tract infections (LRTIs) remain a leading cause of mortality worldwide. Rapid and comprehensive etiological diagnosis is critical yet challenging: existing approaches, from culture to multiplex PCR and mNGS, carry distinct limitations in one or more of turnaround time, sensitivity, cost, or quantitati...