Claudia Ortiz-Calderón

Establishment of an in vitro culture and regeneration protocol for the native Chilean grass Polypogon australis Brong

Polypogon australis Brong. is a native Chilean grass frequently found colonizing metal-rich mine tailings, yet it lacks an established in vitro regeneration system to support controlled physiological and biotechnological studies. Here, we report a reproducible protocol for seed germination, callus induction, and plant...