Glyoxyl-agarose immobilized Bacillus subtilis chitosanase as a reusable biocatalyst for modulated production of chitooligosaccharides.
Enzymatic production of low-molecular-weight chitosan and chitooligosaccharides (COS) with consistent properties is challenging since chitosanases are unstable under acidic, high-temperature conditions. To address this, a recombinant chitosanase was covalently attached to glyoxyl-activated agarose via multipoint attach...