Colin W. Levy

Protein-confined imidazolium intermediates enable diverse biocatalytic C–C and C–N bond formations

Developing enzymatic strategies to selectively construct C–C and C–heteroatom bonds is a major objective in modern biocatalysis. Here we combine genetic code reprogramming and laboratory evolution to establish a family of allylic transferase enzymes that can achieve a remarkable breadth of chemistry, enabling the gener...