Enhanced resistance of pea starch and cake to retrogradation and digestion by mutant 1,4-α-glucan branching enzyme from Bifidobacterium longum.
The 1,4-α-glucan branching enzyme found in Bifidobacterium longum can modify the physicochemical properties of starch by forming denser and shorter branches. To enhance its activity and modification function with regard to pea starch, ten mutants were engineered using site-directed mutagenesis based on multiple sequenc...