Rapid CRISPR-based bovine embryo sexing to streamline genotype-informed cattle breeding.
An efficient, low-resource workflow for bovine embryo sexing that combines whole genome amplification (WGA) with recombinase polymerase amplification-CRISPR-Cas12a (RPA-Cas12a) is developed and could reduce unnecessary cryopreservation and genotyping while improving the efficiency of genotype-informed cattle breeding p...