Hydroxamate formation in Thermochelin Biosynthesis.
Nitrogen-hydroxylating monooxygenases (NMOs) are key enzymes in siderophore biosynthesis that rely on coordinated protein and cofactor conformational changes for catalysis. Here, we characterize TheA, an NMO from Thermocrispumagreste. Steady-state kinetics of TheA reveal a substrate preference for L-ornithine over L-ly...