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Review Open access Sep 2026

Large serine recombinase-mediated gene insertion for high-throughput screens: advantages, design principles, and applications

Abstract High-throughput functional assays, such as multiplexed assays of variant effect (MAVE), increasingly demand stable, precise integration of large DNA libraries into mammalian genomes. While CRISPR-based technologies excel at localized, small-scale edits, they are constrained by payload size limits, heterogeneou...

A. Martella, Kenneth A. Matreyek, D. Fisher · 0 citations

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