Dali Li

Precise DNA base editing using AlphaFold3-based contact modelling

Ultra-sensitive profiling of CRISPR-Cas off-target effects with Tracking-seq2

Track-seq2 provides a robust platform for sensitive off-target detection in primary cells, with sensitivity comparable to or exceeding current state-of-the-art methods.

High-diversity base mutagenesis via simultaneous adenine, cytosine and guanine editing

Base editors hold great promise in endogenous mutagenesis for genetic screening. However, the development of base editors that induce saturated multi-base conversions with diverse mutation spectrum is challenging. Here, we develop triple base editors (smACGs) that simultaneously mutagenize adenine, cytosine, and guanin...