Dan-Yao Zhou

Optimizing acid stability and catalytic activity of Aspergillus flavus uricase (AfUOX) via surface charge engineering and B-factor guided design.

Uricase with improved acid stability is desirable for biomedical and biotechnological applications, yet enhancing the intrinsic acid tolerance of the enzyme while maintaining high catalytic activity remains a challenge. Here, we employed an integrated rational design strategy combining surface charge optimization and B...