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Daniel A. Fletcher

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Open access Aug 2026

LUCas: Light-Uncaged Cas13a using photocleavable interfering guide RNAs

Abstract CRISPR diagnostics enable sensitive detection of infectious diseases, with the RNA endonuclease Cas13a providing specific, amplification-free RNA detection through collateral trans-cleavage of fluorescent reporters. However, background cleavage from unbound enzyme, contaminating nucleases, and unsynchronized i...

Carlos F. Ng, Deepak Krishnamurthy, Andres Dextre et al. · 0 citations
Open access Jul 2026

Bead-based RNA Detection with Cas13a: Multi-scale Design and Validation

A multi-scale analytical framework integrating nanoscale enzyme kinetics, microscale diffusion constraints, and macroscale assay parameters is presented that is used to develop a bead-based Cas13a assay where the bead becomes fluorescent when the target RNA for that bead is detected.

A. Bhuiya, Siddhansh Agarwal, Carlos F. Ng et al. · 0 citations

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