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Sponsored Design Multi-Agent AI Systems Using MCP and A2A: Engineer your own Pyt Design Multi-Agent AI Systems Using MCP and A2A: Engineer your own Pyt

Author

Daniel G. Isom

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Open access Jul 2026

Protocol for CRISPR genome editing in S. cerevisiae using PCR-based guide insertion

Summary Here, we present a CRISPR-Cas9 genome-editing protocol for Saccharomyces cerevisiae that simplifies guide construction and enables rapid guide iteration by replacing restriction/ligation cloning with PCR-based guide installation and seamless Cas9-plasmid recircularization. We describe single-guide RNA (sgRNA) a...

H. Rostamian, Ethan Madden, Frank M. Kaplan et al. · 0 citations

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