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Sponsored AI Networking Cookbook: Practical recipes for AI-assisted network automation and development AI Networking Cookbook: Practical recipes for AI-assisted network automation and development

Author

Davide Mazzeo

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Open access

An Electroporation-Based Protocol for Ex Vivo Base Editing: From Design to Quantitative Assessment.

Base editing is a CRISPR variant approach that enables single-nucleotide conversions without generating double-strand breaks. Cytosine and adenine base editors mediate C•G to T•A and A•T to G•C transitions, respectively, by coupling a deaminase to a catalytically impaired Cas9, a modified nuclease that lacks DNA cleava...

Irene Peña-Gutiérrez, Davide Mazzeo, A. Bassons-Bascuñana et al. · 0 citations
Open access Aug 2026

Adenine base editing correction of LMNA c.745C>T (p.R249W) in congenital muscular dystrophy myoblasts improves cellular phenotype while revealing deleterious p.L248P bystander effects

These results demonstrate that ABE can effectively target the LMNA c.745C>T mutation but also reveal the significant impact of bystander edits on cellular physiology, underscoring the necessity of precise editing technologies to ensure both efficacy and safety in future clinical translation.

M. Santafé, I. Hernández, D. Mazzeo et al. · 0 citations

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