Open access
Oct 2026
GaussF: An Unbiased Gaussian Framework for High-Precision Transcript Quantification with a Programmable Self-Benchmark Model
Short-read RNA sequencing has revolutionized transcriptome analysis, but accurate isoform-level quantification faces substantial challenges from overlapping sequences among complex isoforms and inherent sequencing biases, leading to uneven coverage. Current alignment-based and alignment-free methods, while valuable, la...
Qiang Su, Yi Long, De-Ming Gou et al.
· bioRxiv · 0 citations