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Author

Deepak Krishnamurthy

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Open access Aug 2026

LUCas: Light-Uncaged Cas13a using photocleavable interfering guide RNAs

Abstract CRISPR diagnostics enable sensitive detection of infectious diseases, with the RNA endonuclease Cas13a providing specific, amplification-free RNA detection through collateral trans-cleavage of fluorescent reporters. However, background cleavage from unbound enzyme, contaminating nucleases, and unsynchronized i...

Carlos F. Ng, Deepak Krishnamurthy, Andres Dextre et al. · 0 citations

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