Open access
Sep 2026
Self-amplifying RNA enables rapid, durable, integration-free programming of hiPSCs.
It is shown that a single transfection of saRNA-encoded jRCaMP1b in hiPSCs generates sustained reporter expression throughout differentiation to 3D cardiac spheroids, allowing the tracking of cardiomyocyte function and drug responses.
Catherine M. Della Santina, Deon S. Ploessl, Nicole Lindsay-Mosher et al.
· Cell Reports Methods · 0 citations