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Sponsored Artificial Intelligence: A Modern Approach, Global Edition Artificial Intelligence: A Modern Approach, Global Edition

Author

Diego Rojas

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Open access Aug 2026

Toward ultracentrifugation-free processing and long-term preservation of outer membrane vesicles by culture optimization, tangential flow filtration, diafiltration, and lyophilization

Outer membrane vesicles (OMVs) are bacterial membrane nanoparticles with applications in host–microbe interaction studies, vaccines, molecular delivery, and microbial biotechnology. However, OMV workflows often rely on ultracentrifugation, limiting accessibility and process integration. Here, we evaluated a laboratory-...

Andres Silva, Ignacio Fuentes, Jessenia C. Melio et al. · 0 citations
Jul 2026

A Dictyostelium discoideum Cell-Based Biosensor Reveals Capsule-Associated Virulence Phenotypes in Hypervirulent Klebsiella pneumoniae.

A proof-of-concept cell-based biosensing framework using the social amoeba Dictyostelium discoideum to detect capsule-associated virulence phenotypes in the model hvKp strain SGH10 is established, showing that loss of capsule production strongly reduces mucoviscosity, zebrafish lethality, resistance to amoebal predatio...

Ian Pérez-Ramírez, Matías Gálvez-Silva, Diego Rojas et al. · 0 citations

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