This work demonstrates an integrated metabolic engineering strategy combining pathway reconstruction, physiological stress mitigation, model-guided target selection, and multilevel expression balancing for developing S. oneidensis as a porphyrin-producing cell factory.
Dan Sun, Jie Wu, Ru-Li He et al.· Metabolic Engineering· 0 citations
Biological pollution, including pathogenic microorganisms, antibiotic resistance, invasive species, and harmful organisms, poses increasing threats to ecosystems, food security, and public health. In recent years, CRISPR-based technologies have emerged as powerful tools for biological pollution control due to their hig...