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Dongsheng Ren

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Jul 2026

Transcriptomic Profiling Identifies MyD88 and Pik3r3 as Hub Genes Associated with the Anti-hypertrophic Effects of B-type Natriuretic Peptide.

INTRODUCTION B-type natriuretic peptide (BNP) is widely used to diagnose and assess heart failure prognosis. However, its effects on pathological cardiomyocyte hypertrophy and the underlying mechanisms remain largely unexplored. METHODS Here, we report the use of isoproterenol (ISO)-induced cardiomyocyte hypertrophy models with varying doses of BNP to investigate the potential targets and molecular mechanisms of BNP in rat cardiomyocyte hypertrophy. RESULTS Phenotypic measurement confirmed that BNP treatment significantly attenuated ISOinduced cardiomyocyte hypertrophy. Transcriptomic profiling of cardiomyocytes identified key markers and pathways associated with hypertrophy and BNP intervention, revealing that BNP could broadly reverse ISO-induced molecular perturbations. Trajectory analysis and network module analysis further highlighted MyD88 and Pik3r3 as candidate regulatory nodes associated with BNP intervention. BNP treatment potentially downregulates these genes, which is accompanied by reduced activity of inflammatory and growth-related signaling networks. These changes are associated with pathways involved in sarcomeric protein synthesis, immune-inflammatory responses, myocardial fibrosis, mitochondrial homeostasis, and oxidative stress, suggesting a potential role for BNP in attenuating cardiomyocyte hypertrophy. DISCUSSION These findings suggest that BNP may exert protective effects against cardiomyocyte hypertrophy through coordinated modulation of multiple signaling pathways. MyD88- and Pik3r3- associated networks emerged as potential molecular mechanisms underlying BNP-mediated responses and may represent promising targets for future investigation. CONCLUSION This study provides transcriptomic evidence that BNP may attenuate cardiomyocyte hypertrophy and identifies MyD88- and Pik3r3-associated signaling networks as potential molecular mediators of this response. These findings suggest possible mechanisms underlying the protective effects of BNP and provide a foundation for future functional validation studies in hypertrophic heart disease.

Changchun Wu, Yaqing Wang, Zixuan Zhang et al. · 0 citations
Jul 2026

Time-resolved transcriptomics reveals ABA-related regulation and phenylpropanoid responses in Herpetospermum pedunculosum roots under salt stress.

Salt stress is a major abiotic factor limiting plant growth and productivity. Herpetospermum pedunculosum, a medicinal plant adapted to high-altitude environments, offers a unique non-model system for investigating salt stress responses. Here, we combined physiological and biochemical assays with time-course root transcriptome profiling to characterize the responses of H. pedunculosum to 200 mM NaCl treatment. Salt stress induced rapid wilting, oxidative stress-related physiological changes, membrane damage, and significant accumulation of ABA, total lignin, and total lignans. RNA-seq across five time points (1, 3, 24, 48, and 96 h) identified nearly 28,000 differentially expressed genes (DEGs), which formed distinct temporal clusters associated with hormone signaling, transcriptional regulation, stress responses, and phenylpropanoid-related metabolism. Antioxidant enzyme-related genes, ABA biosynthesis/homeostasis genes, ABA signaling components, and lignin/lignan pathway biosynthesis genes (LLPBGs) showed stage-specific expression patterns, with several genes responding rapidly during the early phase of salt stress. WGCNA and co-expression analyses further identified trait-associated modules and candidate links among core TFs, ABA-related genes, and LLPBGs. Subcellular localization, yeast transactivation, Y1H and Dual-LUC assays provided preliminary evidence that the AP2/ERF factor HpERF141 can bind to and activate the promoter of the previously characterized lignan-related gene HpDIR17. Overall, this study provides a root-focused transcriptomic resource and identifies candidate regulatory genes potentially linking ABA-related responses with lignin/lignan-associated metabolism in H. pedunculosum under salt stress.

Yang Tao, Xiao Huang, Enhao Zhang et al. · 1 citation