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Dongzhi Yang

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Aug 2026

A dual-signal lateral flow biosensing platform for visual detection of miRNA-21 in serum.

Herein, we report a rationally designed dual-mode lateral flow assay (LFA) biosensor for the visual detection of microRNA-21 (miRNA-21), a critical breast cancer biomarker. The platform utilizes a synergistic readout of colorimetric signals from gold nanoparticles (AuNPs) and fluorescent signals from quantum dot nanobeads (QDNBs). Upon the specific introduction of target miRNA-21, the conformation of optimized hairpin nucleic acid probes conjugated on the nanomaterials is disrupted. This structural transition exposes capture sites, generating an AuNP-mediated colorimetric signal and a restored QDNBs fluorescence signal on the test lines by mitigating the inner filter effect (IFE) between them. Compared with conventional single-signal or amplification-dependent miRNA sensors, the proposed LFA integrates amplification-free target recognition, complementary AuNP-based colorimetric and QDNB-based fluorescent readouts, and IFE-regulated fluorescence recovery on a single strip, enabling visual screening and smartphone-assisted semi-quantitative analysis without complex instrumentation. Under optimal conditions, both the colorimetric and fluorescent readouts display broad linear correlations with miRNA-21 concentrations (5-1000 nM and 2.5-1000 nM, respectively), the limits of detection (LOD) of 1.599 nM and 1.354 nM. Furthermore, the platform demonstrates remarkable specificity against homologous miRNAs and robust stability. In spiked human serum, the assay achieves satisfactory recovery rates (91.8%-113.2%) with relative standard deviations (RSDs) below 6.0%. Furthermore, a preliminary evaluation using serum samples from five breast cancer patients and five healthy individuals showed clearly distinguishable colorimetric and fluorescent responses between the two groups, supporting the effectiveness of the proposed probe for miRNA-21 detection in real serum samples. These results indicate the potential of the dual-signal LFA platform for further clinical application.

Ruien Shi, Qian Xiang, Yajuan Chang et al. · 0 citations
Aug 2026

A Tetrazine-Metal-Polyphenol Nanoprobe for Single-Tube Multimodal Sulfide Analysis.

Reliable sulfide analysis in complex matrices remains challenging because single-signal probes are susceptible to interference and ambiguous readouts. Here, we report a tetrazine-metal-polyphenol nanoprobe, RhB@Tz-MPN, for a single-tube sequential multimodal workflow for sulfide analysis. Sulfide produces three role-differentiated responses: a tetrazine-associated UV-vis blue shift for rapid screening, fluorescence recovery accompanying framework reorganization for quantitative readout, and suppression of peroxidase-like activity as a substrate-dependent cross-checking signal. All three readouts are obtained sequentially from the same sample tube, reducing sample transfer and intertube variation. Exploratory residual analysis showed a low observed residual correlation between the two direct optical channels (r = 0.06), while the nanozyme response was more appropriately treated as a sequential cross-checking readout. Under the acidic workflow condition, the fluorescence channel showed a linear range of 5-50 μM with an LOD of 0.79 μM. Spike-and-recovery experiments in tap water and lake water gave recoveries of 102.50-118.16% with RSDs below 4%. The platform also responded to stimulation-induced, cell-associated H2S generation in HepG2 cells and differentiated accumulated spoilage-associated headspace exposure from pork. This work establishes a role-differentiated single-tube strategy for integrating screening, quantification, and cross-checking in sulfide analysis.

Yufeng Liu, Cong Li, Xiaolong Wang et al. · 0 citations